Overexpression of Enzymatically Active Human Cytosolic and Mitochondrial Thioredoxin Reductase in HEK-293 Cells
نویسندگان
چکیده
منابع مشابه
Overexpression of the human 2-oxoglutarate carrier lowers mitochondrial membrane potential in HEK-293 cells: contrast with the unique cold-induced mitochondrial carrier CGI-69.
Using differential mRNA expression analysis, a previously uncharacterized gene was found to be up-regulated 2-fold in brown adipose tissue (BAT) of mice exposed to cold (4 degrees C) for 48 h. Contig and homology analysis revealed that the gene represents the murine orthologue to a sequence from a public database encoding a putative human protein (CGI-69). The presence of mitochondrial carrier ...
متن کاملMinimal HER1 and HER2 expressions in CHO and HEK-293 cells cause them appropriate negative cells for HERs-related studies
Background: Understanding of cellular and molecular mechanisms involved in cell/tumor growth and progression has led to molecular-targeted therapy. HER1 and HER2 are two main oncogenic components of HER-receptor family considered as targets for cancer research and therapy. Reliable results of HER- related research and targeted therapies dependent upon cell line with known HER expression status....
متن کاملCharacterization of alternative cytosolic forms and cellular targets of mouse mitochondrial thioredoxin reductase.
Thioredoxin reductase (TR) and thioredoxin (Trx) define a major cellular redox system that maintains cysteine residues in numerous proteins in the reduced state. Both cytosolic (TR1 and Trx1) and mitochondrial (TR3 and Trx2) enzymes are essential in mammals, but the function of the mitochondrial system is less understood. In this study, we characterized subcellular localization of three TR3 for...
متن کاملO 2 . 2 The role of thioredoxin reductase in the regulation of migration and gap junctional intercellular communication in HEK - 293 cells
Endocytosis is a process common for all eukaryotic cells, enabling uptake (internalization) of extracellular molecules into membrane-bound vesicles budding from the plasma membrane. Such cargo-loaded vesicles are actively transported on cytoskeletal tracks inside the cell, undergoing fusion and fission between various endosomal compartments in the processes regulated by the SNARE proteins, Rab ...
متن کاملThe ethics of HEK 293.
1 Congregation for the Doctrine of the Faith, Donum vitae (February 2, 1987), Introduction, 5. 2 Ibid., I, 1. The topic of embryonic stem cells is very much in the forefront of public interest. The moral stance on obtaining stem cells from embryos is clear: The Church has always taught that all human life from the very beginning must be respected and protected. If human embryos are killed to ob...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2004
ISSN: 0021-9258
DOI: 10.1074/jbc.m408494200